Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.13/804286

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Title
Simultaneous measurement of intracellular nitric oxide and free calcium levels in chordate eggs demonstrates that nitric oxide has no role at fertilization
Author/Creator
Hyslop, Louise A.;  Carroll, Michael;  Nixon, Victoria L.;  McDougall, Alex;  Jones, Keith T.
Description
At fertilization in sea urchin, the free radical nitric oxide (NO) has recently been suggested to cause the intracellular Ca²⁺ rise responsible for egg activation. The authors suggested that NO could be a universal activator of eggs and the present study was set up to test this hypothesis. Intracellular NO and Ca²⁺ levels were monitored simultaneously in eggs of the mouse or the urochordate ascidian Ascidiella aspersa. Eggs were either fertilized or sperm extracts microinjected. Sperm-induced Ca²⁺ rises were not associated with any global, or local, change in intracellular NO, although we were able to detect NO produced by the addition of a NO donor. Furthermore, the NO synthase inhibitor NG-nitro-image-arginine methyl ester had no effect on sperm-induced Ca²⁺ release but did block completely ionomycin-induced NO synthase activation. Therefore, we suggest that the current data provide evidence that NO has no role in the fertilization of these two chordate eggs.
Relation
Developmental Biology Vol. 234, Issue 1, p. 216-230
Publisher Link
http://dx.doi.org/10.1006/dbio.2001.0252
Date
2001
Publisher
Academic Press
Keyword(s)
fertilization;  egg;  mouse;  nitric oxide;  calcium;  mammal;  ascidian;  sea urchin
Resource Type
journal article
Identifier
http://hdl.handle.net/1959.13/804286
Identifier
ISSN:0012-1606
Language
eng
Reviewed
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Subject
"Developmental Biology"
 
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