Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.13/804248
- Calmodulin-dependent protein kinase II triggers mouse egg activation and embryo development in the absence of Ca²⁺ oscillations
Knott, Jason G.;
Gardner, Allison J.;
Jones, Keith T.
- Fertilization in mammalian eggs is accompanied by oscillatory changes in intracellular Ca²⁺ concentration, which are critical for initiating and completing egg activation events and the developmental program. Ca²⁺/Camodulin-dependent protein kinase II (CaMKII) is a multifunctional enzyme that is postulated to be the downstream transducer of the Ca²⁺ signal in many cell types. We tested the hypothesis that CaMKII is the major integrator of Ca²⁺-induced egg activation events and embryo development by microinjecting a cRNA that encodes a constitutively active (Ca²⁺-independent) mutant form of CaMKII (CA-CaMKII) into mouse eggs. Expression of this cRNA, which does not increase intracellular Ca²⁺, induced a sustained rise in CaMKII activity and triggered egg activation events, including cell cycle resumption, and degradation and recruitment of maternal mRNAs; cortical granule exocytosis, however, did not occur normally. Furthermore, when mouse eggs were injected with sperm devoid of Ca²⁺-releasing activity and activated with either CA-CaMKII cRNA or by SrCl₂,similar rates and incidence of development to the blastocyst stage were observed. These results strongly suggest that CaMKII is a major integrator of the Ca²⁺ changes that occur following fertilization.
- Developmental Biology Vol. 296, Issue 2, p. 388-395
- Publisher Link
- Academic Press
- Resource Type
- journal article