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Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.13/804172
- Degradation of APCcdc²⁰ and APCcdh¹ substrates during the second meiotic division in mouse eggs
Jones, Keith T.
- Metaphase II-arrested mouse eggs are stimulated to complete meiosis by sperm-induced Ca²⁺ spiking. The Ca²⁺ signal causes activation of the E3 ligase anaphasepromoting complex/cyclosome (APC), leading to the destruction of key proteins necessary for meiotic exit. We show, using western blots of mouse eggs, the presence of both APC activators cdc20 and cdh1, which target Dbox and D-box/KEN-box substrates, respectively, for proteolysis. We decided to examine the temporal activation of APCcdc²⁰ and APCcdh¹ by coupling APC substrates to GFP and examining their destruction in real-time following release from second meiotic division arrest. D-box substrates were degraded quickly after the initiation of sperm-induced Ca²⁺ spiking, such that their degradation was complete by the time of second polar body extrusion. By contrast, KEN-box-containing substrates were degraded when CDK1 activity was low, during the period between polar body extrusion and pronucleus formation. This observation of apparent APCcdh¹ activity in meiosis II based on destruction of exogenous GFP-coupled substrates was then confirmed by observing destruction of endogenous APCcdh¹ substrates. These data are consistent with a model of initial APCcdc²⁰ activation on sperminduced activation, followed by APCcdh¹ activation after second polar body extrusion.Interestingly,therefore, we propose that mammalian eggs undergo meiosis II with both APCcdc²⁰ and APCcdh¹, whereas eggs of other species so far described have APCcdc²⁰ activity only.
- Journal of Cell Science Vol. 117, Issue 26, p. 6289-6296
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cell cycle proteins;
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