Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.13/43162

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Title
The rat sperm proteome characterized via IPG strip prefractionation and LC-MS/MS identification
Author/Creator
Baker, Mark A.;  Hetherington, Louise;  Reeves, Gabi;  Muller, Jorg;  Aitken, R. John
Institution
The University of Newcastle. Faculty of Science & Information Technology, School of Environmental and Life Sciences
Description
Proteomics represents a powerful tool for the analysis of mammalian spermatozoa, since these terminally differentiated cells are transcriptionally inactive and exhibit a limited dynamic range of protein expression. Here we report the identification of 5123 peptides, leading to 829 unambiguous and 2215 redundant gene products found to be present within rat spermatozoa derived from the cauda epididymis. Bioinformatics demonstrated that 60 proteins appeared to be specifically expressed in the genitourinary tract, including pyruvate dehydrogenase 1, ropporin, testis-specific serine kinase 4, testis-specific transporter, and retinol dehydrogenase 14. We also identified eight members of the ADAM family, seven of which have previously been detected in spermatozoa (ADAM2, -3, -4, -5, -6, -7, and -30) while ADAM34 has been identified in the sperm proteome for the first time. Approximately 21 gene products were found to possess isomerase activity including peptidylprolyl cis/trans isomerases that are known to be involved in germ cell differentiation and protein disulfide isomerases that have been implicated in sperm-oocyte fusion. Furthermore, 51 gene products clustered into ion-transporter activity. This inventory of gene products, the first ever 2-D LC-MS/MS analysis of rat spermatozoa, will be invaluable in directing future research into the molecular mechanisms that drive these highly specialized cells.
Relation
Proteomics Vol. 8, Issue 11, p. 2312-2321
Publisher Link
http://dx.doi.org/10.1002/pmic.200700876
Date
2008
Publisher
Wiley - VCH Verlag GmbH & Co. KGaA
Keyword(s)
immobilized pH gradients;  protein profile;  proteosome;  shotgun proteomics;  spermatogenesis
Resource Type
journal article
Identifier
http://hdl.handle.net/1959.13/43162
Identifier
ISSN:1615-9853
Reviewed
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Subject
"Proteomics"
 
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