Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.13/43001
- Chlamydia muridarum infection subverts dendritic cell function to promote Th2 immunity and airways hyperreactivity
Kaiko, Gerard E.;
Hickey, Danica K.;
Lam, Chuan En;
Hansbro, Philip M.;
Foster, Paul S.;
Beagley, Kenneth W.
- The University of Newcastle. Faculty of Health, School of Biomedical Sciences and Pharmacy
- There is strong epidemiological evidence that Chlamydia infection can lead to exacerbation of asthma. However, the mechanism(s) whereby chlamydial infection, which normally elicits a strong Th type 1 (Th1) immune response, can exacerbate asthma, a disease characterized by dominant Th type 2 (Th2) immune responses, remains unclear. In the present study, we show that Chlamydia muridarum infection of murine bone marrow-derived dendritic cells (BMDC) modulates the phenotype, cytokine secretion profile, and Ag-presenting capability of these BMDC. Chlamydia-infected BMDC express lower levels of CD80 and increased CD86 compared with noninfected BMDC. When infected with Chlamydia, BMDC secrete increased TNFα, IL-6, IL-10, IL-12, and IL-13. OVA peptide-pulsed infected BMDC induced significant proliferation of transgenic CD4⁺ DO11.10 (D10) T cells, strongly inhibited IFNγ secretion by D10 cells, and promoted a Th2 phenotype. Intratracheal transfer of infected, but not control noninfected, OVA peptide-pulsed BMDC to naive BALB/c mice, which had been i.v. infused with naive D10 T cells, resulted in increased levels of IL-10 and IL-13 in bronchoalveolar lavage fluid. Recipients of these infected BMDC showed significant increases in airways resistance and decreased airways compliance compared with mice that had received noninfected BMDC, indicative of the development of airways hyperreactivity. Collectively, these data suggest that Chlamydia infection of DCs allows the pathogen to deviate the induced immune response from a protective Th1 to a nonprotective Th2 response that could permit ongoing chronic infection. In the setting of allergic airways inflammation, this infection may then contribute to exacerbation of the asthmatic phenotype.
- Journal of Immunology Vol. 180, Issue 4, p. 2225-2232
- American Association of Immunologists
genital tract infection;
- Resource Type
- journal article