Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.13/41351

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Title
Molecular architecture of the 'stressosome,' a signal integration and transduction hub
Author/Creator
Marles-Wright, Jon;  Grant, Tim;  Rohou, Alexis;  Tichelaar, Willem;  van Heel, Marin;  Lewis, Richard J.;  Delumeau, Olivier;  van Duinen, Gijs;  Firbank, Susan J.;  Lewis, Peter J.;  Murray, James W.;  Newman, Joseph A.;  Quin, Maureen B.;  Race, Paul R.
Institution
The University of Newcastle. Faculty of Science & Information Technology, School of Environmental and Life Sciences
Description
A commonly used strategy by microorganisms to survive multiple stresses involves a signal transduction cascade that increases the expression of stress-responsive genes. Stress signals can be integrated by a multiprotein signaling hub that responds to various signals to effect a single outcome. We obtained a medium- resolution cryo- electron microscopy reconstruction of the 1.8- megadalton "stressosome" from Bacillus subtilis. Fitting known crystal structures of components into this reconstruction gave a pseudoatomic structure, which had a virus capsid-like core with sensory extensions. We suggest that the different sensory extensions respond to different signals, whereas the conserved domains in the core integrate the varied signals. The architecture of the stressosome provides the potential for cooperativity, suggesting that the response could be tuned dependent on the magnitude of chemophysical insult.
Relation
Science Vol. 322, Issue 5898, p. 92-96
Publisher Link
http://dx.doi.org/10.1126/science.1159572
Date
2008
Publisher
American Association for the Advancement of Science
Keyword(s)
transcription factor sigma (B);  Bacillus-subtilis;  environmental stress;  Serine Kinase;  pathway;  protein;  complex;  activation;  RsbR;  phosphorylation
Resource Type
journal article
Identifier
http://hdl.handle.net/1959.13/41351
Identifier
ISSN:0036-8075
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Reviewed
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Subject
"Science"
 
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