Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.13/29234

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Title
The midcell replication factory in Bacillus subtilis is highly mobile: implications for coordinating chromosome replication with other cell cycle events
Author/Creator
Migocki, Margaret D.;  Lewis, Peter John;  Wake, R. Gerry;  Harry, Elizabeth J.
Description
During vegetative growth, rod-shaped bacterial cells such as Escherichia coli and Bacillus subtilis divide precisely at midcell. It is the Z ring that defines the position of the division site. We previously demonstrated that the early stages of chromosome replication are linked to midcell Z ring assembly in B. subtilis and proposed a direct role for the centrally located replication factory in masking and subsequently unmasking the midcell site for Z ring assembly. We now show that the replication factory is significantly more scattered about the cell centre than the Z ring in both vegetative cells and outgrown spores of B. subtilis. This finding is inconsistent with the midcell replication factory acting as a direct physical block to Z ring assembly. Time-lapse experiments demonstrated that the lower precision of replication factory positioning results from its high mobility around the cell centre. Various aspects of this mobility are presented and the results are discussed in the light of current views on the determinants of positional information required for accurate chromosome segregation and cell division.
Relation
Molecular Microbiology Vol. 54, Issue 2, p. 452-463
Relation
http://www3.interscience.wiley.com/journal/118790124/abstract
Date
2004
Publisher
Wiley-Blackwell Publishing Ltd.
Keyword(s)
midcell replication;  bacillus subtilis;  mobile;  chromosome;  replication
Resource Type
journal article
Identifier
http://hdl.handle.net/1959.13/29234
Identifier
ISSN:0950-382X
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Subject
"Molecular Microbiology"
 
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