Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.13/27756
- Rabbit epididymal secretory proteins. I. Characterization and hormonal regulation
Jones, Russell C.;
Hansen, Lyall A.;
Holland, Michael K.
- Analyses of samples of luminal fluid from the rete testis, distal efferent ducts, and epididymal regions 2–5 and 8 revealed that 91% of the fluid leaving the testis is reabsorbed by the efferent ducts, 79% of the remainder is reabsorbed proximal to epididymal regions 4 and 5, and there is a net secretion of fluid into the duct caudally. There is a net reabsorption by the efferent ducts of 73% of the protein leaving the testis and then a net secretion along the epididymis. SDS-PAGE of the luminal fluids indicated that four new protein bands that were not present in blood appeared in the efferent ducts, 5 in epididymal regions 1–5, 6 in regions 6 and 7, and one in region 8. Two bands in samples from the efferent ducts were absent caudally, and one band present in region 7 was absent in region 8. The rates of incorporation of ³₅S-methionine into minced duct in vitro varied among regions when expressed per milligram of wet weight of tissue (region 2–5 > region 7 > region 6 > region 1 > region 8 > ductuli efferentes), and orchidectomy had little effect on the rates. Incorporation into four proteins that were secreted in vitro (Mr 38 000, 20 000, 15 000, and 13 000) was reduced or abolished by orchidectomy and restored by testosterone therapy. The secretion of three proteins (Mr 52 000, 23 000, and 22 000) was reduced or abolished by orchidectomy and not restored by testosterone therapy. SDS-PAGE of detergent extracts of sperm indicated that five proteins were lost and nine were gained during epididymal transit. Seven of the proteins gained were about the same molecular weight as proteins secreted by the epididymis (Mr 94 000, 52 000, 38 000, 36 000, 22 000, 20 000, and 13 000) and were analyzed using N-terminal amino acid microsequencing.
- Biology of Reproduction Vol. 67, Issue 1, p. 133-139
- Society for the Study of Reproduction,Inc
- Resource Type
- journal article