Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.13/27385

OpenURL Link
Title
The efficacy of Propionibacterium jensenii 702 to stimulate a cell-mediated response to orally administered soluble Mycobacterium tuberculosis antigens using a mouse model
Author/Creator
Adams, Michelle C.;  Lean, Melanie L.;  Hitchick, Nola C.;  Beagley, Kenneth W.
Description
Mucosal vaccination using adjuvant protein vaccines may offer a novel approach for tuberculosis. To date, however, development of such a vaccine has been considered unlikely due to the inability to identify a safe adjuvant that stimulates an appropriate immune response. This study was undertaken to determine the potential of a dairy propionibacteria, P. jensenii strain 702 (PJ702), to act as an adjuvant when co-administered orally with soluble tuberculosis protein to mice. The efficacy of the PJ702 to act as an adjuvant was assessed by comparison with cholera toxin. C57 mice were orally immunized with Mycobacterium tuberculosis short-term culture filtrate protein (STCF) (200 μg) with either PJ702 (10⁸ cfu) or cholera toxin (10 μg) in a total volume of 100 $mu$L. A control group was given PJ702 10⁸ cfu alone. Each mouse (eight per group) was vaccinated weekly over a 21-day period. At day 25 the mice were sacrificed, spleens were collected and lymphocyte cultures prepared. After stimulation with STCF (2.5 μg), cell proliferation was measured by ³H thymidine uptake, and the cytokines, IL-4 and IFN-γ, by ELISA. A significantly higher T-cell proliferation was observed for the group given the vaccine containing PJ702, compared to both the control group and the group given the vaccine containing cholera toxin (P < 0.05). The predominant cytokine produced from all groups was IFN-γ. These results indicate potential for future development of an oral tuberculosis vaccine, and also identify PJ702 as a potential living vaccine vector that could be applied to a number of mucosally transmitted diseases.
Relation
Le Lait Vol. 85, Issue 39084, p. 75-84
Publisher Link
http://dx.doi.org/10.1051/lait:2005003
Date
2005
Publisher
EDP Sciences
Keyword(s)
Propionibacterium;  tuberculosis;  vaccine;  cell-mediated;  adjuvant
Resource Type
journal article
Identifier
http://hdl.handle.net/1959.13/27385
Identifier
ISSN:0023-7302
Reviewed
Reviewed
Full Text
Full Text
46 Visitors 54 Hits 3 Downloads
Save/E-mail Citation
Citation Format
E-mail Address
Subject
"Le Lait"
 
OR