Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.13/27365

OpenURL Link
Title
Induced sputum 8-isoprostane concentrations in inflammatory airway diseases
Author/Creator
Wood, Lisa G.;  Garg, Manohar L.;  Simpson, Jodie L.;  Mori, Trevor A.;  Croft, Kevin D.;  Wark, Peter A. B.;  Gibson, Peter G.
Description
Induced sputum 8-iso-prostaglandin F₂α (PGF₂α) concentrations may be a useful marker of oxidative stress in airways disease. This study examines oxidative stress (measured by 8-iso-PGF₂α) in airway disease according to disease type (asthma and bronchiectasis), disease activity (stable and acute asthma), and disease pattern (intermittent, mild, moderate, and severe persistent asthma). We compared subjects with stable asthma (n = 71) and bronchiectasis (n = 23) with healthy control subjects (n = 29). Another group of patients with asthma (n = 39) were assessed during and after acute exacerbation. Induced sputum 8-iso-PGF₂α concentrations were validated and found to be elevated in subjects with stable asthma and bronchiectasis versus control subjects (median [interquartile range] 216 [103-389] and 698 [264-1,613] ng/L vs. 123 [41-290] ng/L, p < 0.001) and increased as clinical asthma pattern worsened (intermittent 115 [42-153], mild persistent 116 [89-229] ng/L, moderate persistent 183 [110-317] ng/L, severe persistent 387 [102-587] ng/L; p = 0.010). Sputum 8-iso-PGF₂α concentrations were elevated during acute asthma and decreased with recovery (458 [227-950] ng/L vs. 214 [148-304] ng/L, p = 0.0002). We conclude that 8-iso-PGF₂α is involved in the pathophysiology of inflammatory airway diseases, being related to disease type, pattern, and activity. Analysis of 8-iso-PGF₂α concentrations in induced sputum provides a useful tool for monitoring oxidative stress and investigating strategies aimed at reducing oxidative stress in airways disease.
Relation
American Journal of Respiratory and Critical Care Medicine Vol. 171, Issue 5, p. 426-430
Publisher Link
http://dx.doi.org/10.1164/rccm.200408-1010OC
Date
2005
Publisher
American Thoracic Society
Keyword(s)
isoprostanes;  lipid peroxidation;  oxidative stress
Resource Type
journal article
Identifier
http://hdl.handle.net/1959.13/27365
Identifier
ISSN:1535-4970
Reviewed
Reviewed
25 Visitors 27 Hits 0 Downloads
Save/E-mail Citation
Citation Format
E-mail Address
Subject
"American Journal of Respiratory and Critical Care Medicine"
 
OR