Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.13/27036

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Title
Improved plasmid vectors for the production of multiple fluorescent protein fusions in Bacillus subtilis
Author/Creator
Feucht, Andrea;  Lewis, Peter J.
Description
The intrinsically fluorescent green fluorescent protein has been used in many laboratories as a cytological marker to monitor protein localisation in live cells. Multiple spectrally modified mutant versions and novel fluorescent proteins from other species have subsequently been reported and used for labelling cells with multiple fluorescent protein fusions. In this work we report the design and use of vectors containing some of these spectral variants of GFP for use in the Gram positive bacterium Bacillus subtilis. These vectors complement those previously described (Lewis and Marston, 1999. Gene 227, 101–109) to provide a large suite of plasmid vectors for use in this and other related Gram positive organisms. Using these vectors we have been able to directly demonstrate the sequential assembly/disassembly of proteins involved in the generation of cellular asymmetry during development.
Relation
Gene Vol. 264, Issue 2, p. 289-297
Publisher Link
http://dx.doi.org/10.1016/S0378-1119(01)00338-9
Date
2001
Publisher
Elsevier Science BV
Keyword(s)
fluorescent proteins;  dual labelling;  subcellular localisation;  Bacillus subtilis
Resource Type
journal article
Identifier
http://hdl.handle.net/1959.13/27036
Identifier
ISSN:0378-1119
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Subject
"Gene"
 
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