Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.13/26912

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Title
Safety and immunogenicity of an oral inactivated whole-cell Pseudomonas aeruginosa vaccine administered to healthy human subjects
Author/Creator
Cripps, Allan W.;  Peek, Keith;  Dunkley, Margaret;  Vento, Kevin;  Marjason, Joanne K.;  McIntyre, Madonna E.;  Sizer, Phil;  Croft, Duncan;  Sedlak-Weinstein, Lis
Description
This study examines the safety and immunogenicity of an oral, whole-cell Pseudomonas aeruginosa vaccine administered to healthy volunteers. Thirty subjects received an oral dose of Pseudostat in two timed, measured doses with serological follow-up to 56 days postvaccination. Following vaccination, several individuals were identified as antibody responders for all three immunoglobulin (Ig) isotypes tested, specifically against whole-cell P. aeruginosa extract and outer membrane proteins F and I. The mean pooled lipopolysaccharide antigen-specific IgA showed the most significant and constant increases in titer postdose, with a similar increase in titer for whole-cell P. aeruginosa extract-specific IgA. The results demonstrated an increased phagocytic ability of the selected macrophage cell line in post vaccination sera. Furthermore a significant increase in intracellular macrophage killing of opsonized P. aeruginosa was also demonstrated (82% on day 14 postdose) in the presence of the postvaccination sera. The safety component of the study did not show any vaccine-attributable adverse effects in any of the subjects, as documented by clinical evidence, hematology, and biochemistry profiles. We conclude that Pseudostat is safe and immunogenic in humans at this dose and that further studies to determine the appropriate dosage and efficacy are needed. In our study, we have shown that the most significant and sustained responses to oral vaccination in human adult volunteers were serum IgA levels and that pooled sera collected postimmunization have an increased capacity to promote opsonophagocytotic killing of P. aeruginosa.
Relation
Infection and Immunity Vol. 74, Issue 2, p. 968-974
Publisher Link
http://dx.doi.org/10.1128/IAI.74.2.968-974.2006
Date
2006
Publisher
American Society for Microbiology
Keyword(s)
Pseudomonas aeruginosa;  vaccination;  immunoglobulin isotypes;  macrophage cell;  Pseudostat;  oral vaccines
Resource Type
journal article
Identifier
http://hdl.handle.net/1959.13/26912
Identifier
ISSN:1098-5522
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Subject
"Infection and Immunity"
 
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