Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.13/932994
- Title
- All-trans retinoic acid regulates the expression of the extracellular matrix protein fibulin-1 in the guinea pig sclera and human scleral fibroblasts
- Author/Creator
-
Li, Chuanxu;
McFadden, Sally A.;
Morgan, Ian;
Cui, Dongmei;
Hu, Jianmin;
Wan, Wenjuan;
Zeng, Junwen
- Institution
- The University of Newcastle. Faculty of Science & Information Technology, School of Psychology
- Description
- Purpose: Fibulin-1 (FBLN1) mRNA is expressed in human sclera and is an important adhesion modulatory protein that can affect cell-matrix interactions and tissue remodeling. Scleral remodeling is influenced by all-trans retinoic acid (RA). Our purpose was to confirm the presence of fibulin-1 protein in guinea pig sclera and investigate the effect of RA on the expression of fibulin-1 in guinea pig sclera in vivo and in cultured human scleral fibroblasts (HSFs). Methods: Confocal fluorescence microscopy was used to study fibulin-1 and aggrecan expression and localization in sclera from control guinea pigs and in animals given RA by daily gavage from 4 to 8 days of age. The effects of RA (from 10⁻⁹ to 10⁻⁵ M) on fibulin-1 expression in HSFs were observed by immunohistochemistry and assayed by real-time PCR and western blot analysis. Results: Fibulin-1 protein expression was detected by confocal fluorescence microscopy in guinea pig sclera and in cultured HSFs. Upregulation of fibulin-1 in scleral tissue was observed after feeding with RA. In vitro, the level of Fbln1 mRNA was increased after treatment of HSFs with RA (at concentrations of 10⁻⁸ to 10⁻⁶ M; p<0.001), with a maximum effect at 10⁻⁷ M. Fibulin-1 protein levels were significantly increased after treatment of HSFs with 10⁻⁷ M of RA for 24 or 48 h (p<0.05). Conclusions: Fibulin-1 protein was expressed in guinea pig sclera and cultured HSFs. Expression was regulated by RA, a molecule known to be involved in the regulation of eye growth. Further studies on the role of fibulin-1 in the regulation of eye growth, including during the development of myopia, are therefore warranted.
- Relation
- Molecular Vision Vol. 16, Issue 76-78, p. 689-697
- Relation
- http://www.molvis.org/molvis/v16/a78
- Date
- 2010
- Publisher
- Molecular Vision
- Keyword(s)
-
aggrecan;
eyes;
components;
receptors
- Resource Type
- journal article
- Identifier
- http://hdl.handle.net/1959.13/932994
- Identifier
- ISSN:1090-0535
- Reviewed
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