Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.13/932663
- Title
- pBaSysBioII: an integrative plasmid generating gfp transcriptional fusions for high-throughput analysis of gene expression in Bacillus subtilis
- Author/Creator
-
Botella, Eric;
Fogg, Mark;
Lewis, Peter J.;
van Dijl, Jan Maarten;
Aymerich, Stéphanie;
Wilkinson, Anthony J.;
Devine, Kevin M.;
Jules, Matthieu;
Piersma, Sjouke;
Doherty, Geoff;
Hansen, Annette;
Denham, Emma L.;
Le Chat, Ludovic;
Veiga, Patrick;
Bailey, Kirra
- Institution
- The University of Newcastle. Faculty of Science & Information Technology, School of Environmental and Life Sciences
- Description
- Plasmid pBaSysBioII was constructed for high-throughput analysis of gene expression in Bacillus subtilis. It is an integrative plasmid with a ligation-independent cloning (LIC) site, allowing the generation of transcriptional gfpmut3 fusions with desired promoters. Integration is by a Campbell-type event and is non-mutagenic, placing the fusion at the homologous chromosomal locus. Using phoA, murAA, gapB, ptsG and cggR promoters that are responsive to phosphate availability, growth rate and carbon source, we show that detailed profiles of promoter activity can be established, with responses to changing conditions being measurable within 1 min of the stimulus. This makes pBaSysBioII a highly versatile tool for real-time gene expression analysis in growing cells of B. subtilis.
- Relation
- Microbiology Vol. 156, Issue 6, p. 1600-1608
- Publisher Link
- http://dx.doi.org/10.1099/mic.0.035758-0
- Date
- 2010
- Publisher
- Society for General Microbiology
- Keyword(s)
-
LCA;
live cell array;
LIC;
ligation-independent cloning
- Resource Type
- journal article
- Identifier
- http://hdl.handle.net/1959.13/932663
- Identifier
- ISSN:1350-0872
- Reviewed
7 Visitors
9 Hits
0 Downloads
List Of Titles
