Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.13/932565

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Title
An amperometric biosensor based on glucose oxidase immobilized in a sol-gel polyvinyl alcohol/silica hybrid composite film on a prussian blue modified electrode
Author/Creator
Zuo, Shao-Hua;  Zhao, Hong-Li;  Zhang, Ling-Fan;  Yuan, Hui-Hui;  Lan, Min-Bo;  Lawrance, Geoffrey A.;  Wei, Gang
Institution
The University of Newcastle. Faculty of Science & Information Technology, School of Environmental and Life Sciences
Description
An amperometric glucose biosensor was fabricated by entrapment of glucose oxidase in a composite matrix of silica sol-gel and polyvinyl alcohol physically layered on top of a Prussian blue-modified Pt electrode. This biosensor functions through the detection of hydrogen peroxide produced by the glucose oxidase-catalyzed oxidation of glucose at -0.05 V. The biosensor exhibited high selectivity, and a sensitivity of 3.41 μA/mM, due to a combination of electroreduction of H₂O₂ at the surface of the modified Pt electrode at low potential and a sympathetic microenvironment for enzyme immobilization provided by the silica sol-gel. The biosensor had a linear response to glucose over the range 5.0×10⁻⁵ to 2.5×10⁻³ M, with a response time of less than 8 sec. The detection limit of the biosensor is 4.0 μM, based on a signal-to-noise ratio of 3. The biosensor exhibits satisfactory long-term stability; 85% of its original activity was retained after 30 days of storage in phosphate buffer (pH 6.9; 0.1 M) at 4 °C.
Relation
Advanced Science Letters Vol. 3, Issue 4, p. 476-481
Relation
http://www.aspbs.com/science/contents-science2010.htm#v3n4
Date
2010
Publisher
American Scientific Publishers
Keyword(s)
amperometric glucose biosensor;  biosensors;  prussian blue-modified electrode;  enzyme-based glucose
Resource Type
journal article
Identifier
http://hdl.handle.net/1959.13/932565
Identifier
ISSN:1936-6612
Reviewed
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Subject
"Advanced Science Letters"
 
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