Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.13/931820

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Title
Calcineurin activity is required for the completion of cytokinesis
Author/Creator
Chircop, Megan;  Malladi, Chandra S.;  Lian, Audrey T.;  Page, Scott L.;  Zavortink, Michael;  Gordon, Christopher P.;  McCluskey, Adam;  Robinson, Phillip J.
Institution
The University of Newcastle. Faculty of Health, School of Biomedical Sciences and Pharmacy
Description
Successful completion of cytokinesis requires the spatio-temporal regulation of protein phosphorylation and the coordinated activity of protein kinases and phosphatases. Many mitotic protein kinases are well characterized while mitotic phosphatases are largely unknown. Here, we show that the Ca2⁺- and calmodulindependent phosphatase, calcineurin (CaN), is required for cytokinesis in mammalian cells, functioning specifically at the abscission stage. CaN inhibitors induce multinucleation in HeLa cells and prolong the time cells spend connected via an extended intracellular bridge. Upon Ca2⁺ influx during cytokinesis, CaN is activated, targeting a set of proteins for dephosphorylation, including dynamin II (dynII). At the intracellular bridge, phospho-dynII and CaN are co-localized to dual flanking midbody rings (FMRs) that reside on either side of the central midbody ring. CaN activity and disassembly of the FMRs coincide with abscission. Thus, CaN activity at the midbody plays a key role in regulating the completion of cytokinesis in mammalian cells.
Relation
Cellular and Molecular Life Sciences Vol. 67, Issue 21, p. 3725-3737
Publisher Link
http://dx.doi.org/10.1007/s00018-010-0401-z
Date
2010
Publisher
Birkhaeuser Verlag
Keyword(s)
calcineurin;  cytokinesis;  dephosphorylation;  dynamin II
Resource Type
journal article
Identifier
http://hdl.handle.net/1959.13/931820
Identifier
ISSN:1420-682X
Reviewed
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Subject
"Cellular and Molecular Life Sciences"
 
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